Item too large for volume format

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Here, I should mention that flash/ USB drive I used in this case was formatted with FAT32 partition style using Windows. Following is the message keep appearing while performing copy operation: The item “” can’t be copied because it is too large for the volume’s format.

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I was trying to copy my 4GB ISO file located on Mac OS X to an empty 8GB flash drive, but Mac doesn’t allows me to do so. Due to differences in these file systems, you may face the volume format related issues while performing file operations on a drive, that has been used on either of the OS. We all know that Windows uses NTFS file system, whereas Mac uses FAT32 file system.